Last data update: 24 January 2024 16:39 CET
Plasmid name: pET15b-anti-GspLM-nb4 (LMBP 11252)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Nanobody targeting Pseudomonas aeruginosa type II secretion system protein complex GspLM Erwinia carotovora pectatelyase B cDNA (pelB); signal sequence Histidine tag (His-tag); N-terminal |
| Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pET15b |
| Further information: | The plasmid was constructed by reengineering the multi-cloning site of pET15b, and cloning the anti-GspLM nanobody clone 1XC65 between the NheI and BamHI restriction sites. |
| EMBL Accession number: | - |
| Latest sequence update: | 27/06/2019 |
| Sequence detail: | The sequence of the modified MCS: ccATGGGCAAATACCTATTGCCTACGGCAGCCGCTGGATTGTTATTACTCGCGG CCCAGCCGGCGATGGCCgccCATCATCATCATCATCATgatgaagttgatGGAg ctagcGGAgaatcc |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was constructed by A. Fulara(1)(2) and Prof. Dr S. Savvides(1)(2). 1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
| Plasmid reference: | Fulara et al., bioRxiv (2020), 1-20 [DOI: 10.1101/2020.03.20.999888] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli B BL21 |
| Host reference: | - |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET15b-anti-GspLM-nb4 (LMBP 11252) is available at BCCM/GeneCorner. The plasmid was constructed by A. Fulara and Prof. Dr S. Savvides and was published in Fulara et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.