Last data update: 24 January 2024 16:39 CET
Plasmid name: pET21_PaGH3 (LMBP 12706)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p12706.gb |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
Pseudomonas aeruginosa beta-N-acetylglucosaminidase cDNA (GH3) Histidine tag (His-tag); C-terminal Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Phage f1 origin |
| Host range: | Escherichia coli |
| EMBL Accession number: | - |
| Latest sequence update: | |
| Authenticity test: | The plasmid has been fully sequenced but the NGS data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by J. Franceus(1) and Prof. Dr T. Desmet(1). (1) Center for Synthetic Biology, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pET21_PaGH3 (LMBP 12706) is available at BCCM/GeneCorner. The plasmid was deposited by J. Franceus and Prof. Dr T. Desmet. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.