Last data update: 24 January 2024 16:39 CET
Plasmid name: pETM14 (LMBP 13744)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | - |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Histidine tag (His-tag); N-terminal Human rhinovirus 3C protease cleavage site (PreScission site, PRS); N-terminal Histidine tag (His-tag); C-terminal Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) |
Promoter: | Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac) |
Ribosome binding site: |
- |
Terminator: | Phage T7 gene 10 terminator (T7g10) |
Selection marker: | Kanamycin (kan) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Copy number: | Low copy number |
Host range: | Escherichia coli |
Further information: | The plasmid is used for recombinant protein production in E. coli. |
EMBL Accession number: | - |
Latest sequence update: | 17/02/2023 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Dr Kim Remans(1). It was constructed by Dr A. Geerlof(1). (1) Protein Expression and Purification Core Facility, EMBL Heidelberg, Germany |
Plasmid reference: | - |
Restricted distribution: | - BCCM/MTA adapted by EMBL - Restricted to academic users - Acknowledgment of the depositor in any publication resulting from the use of the MATERIAL - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5αT1R |
Host reference: | - |
Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pETM14 (LMBP 13744) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Kim Remans. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.