Last data update: 24 January 2024 16:39 CET
Plasmid name: pEXPRMB14-GCS-1-GFP (LMBP 9689)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Caenorhabditis elegans gamma glutamylcysteine synthetase 1 cDNA (gcs-1) Aequorea victoria green fluorescent protein DNA (GFP); C-terminal |
| Promoter: | Caenorhabditis elegans ribosomal protein RPL-1 promoter (rpl-1) Escherichia coli lac operon promoter Phage T3 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Caenorhabditis elegans unc-54 terminator (unc-54) |
| Selection marker: | Ampicillin (amp) Caenorhabditis elegans unc-119 (unc-119); auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Caenorhabditis elegans; unc-119(-) |
| Parental clone: | pDestMB14 |
| Further information: | The plasmid was created by cloning the C. elegans gsc-1 coding sequence in frame with the GFP marker in the pDestMB14 vector via Gateway recombination. The nucleotide sequence of the C. elegans inserts can be retrieved from the Genbank accession numbers NC_003280.10, NC_003279.8 and NC_003281.10. Other name of the plasmid is GCS-1:GFP (E.coli DH5 α). |
| EMBL Accession number: | NC_003280.10, view at EMBL, GenBank, DDBJ NC_003279.8, view at EMBL, GenBank, DDBJ NC_003281.10, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/12/2015 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by C. Vlaeminck(1) and Prof. Dr B. Braeckman(1). (1) Research Group for aging physiology and molecular evolution, Department of Biology, Ghent University, Ghent |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEXPRMB14-GCS-1-GFP (LMBP 9689) is available at BCCM/GeneCorner. This plasmid was deposited by C. Vlaeminck and Prof. Dr B. Braeckman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.