Last data update: 24 January 2024 16:39 CET
Plasmid name: pEXPRMB14-noGFP-unc54-CTS-1 (LMBP 9732)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Caenorhabditis elegans mitochondrial citrate synthase 1 cDNA (cts-1) |
Promoter: | Caenorhabditis elegans mitochondrial citrate synthase 1 promoter (cts-1) Escherichia coli lac operon promoter Phage T3 promoter Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | Caenorhabditis elegans unc-54 terminator (unc-54) |
Selection marker: | Ampicillin (amp) Caenorhabditis elegans unc-119 (unc-119); auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli Caenorhabditis elegans; unc-119(-) |
Parental clone: | pDestMB14; pENTRYP4P1R-CTS-1 |
Further information: | This Gateway Expression vector was created by removing the GFP coding sequence from pDestMB14 and cloning the C. elegans cts-1 gene, along with its promoter, into this pDestMB14-derived vector via Gateway recombination with pENTRYP4P1R-CTS-1. The nucleotide sequence of the C. elegans inserts can be retrieved from the Genbank accession numbers NC_003281.10 and NC_003279.8. Other name of the plasmid is pEXPRMB14noGFPunc54UTR cts-1 (10.87.1). |
EMBL Accession number: | NC_003281.10, view at EMBL, GenBank, DDBJ NC_003279.8, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 09/12/2015 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by C. Vlaeminck(1) and Prof. Dr B. Braeckman(1). (1) Research Group for aging physiology and molecular evolution, Department of Biology, Ghent University, Ghent |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pEXPRMB14-noGFP-unc54-CTS-1 (LMBP 9732) is available at BCCM/GeneCorner. This plasmid was deposited by C. Vlaeminck and Prof. Dr B. Braeckman. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.