Last data update: 24 January 2024 16:39 CET
Plasmid name: pFA-HAH1-MET3P (LMBP 11187)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p11187.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Candida albicans sulfate adenylyltransferase promoter (MET3) Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Candida albicans HIS1; auxotrophic Candida albicans ARG4; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Candida albicans; arg4(-), integrative |
| Parental clone: | pFA-HAH1 |
| Further information: | The plasmid was constructed by cloning the C. albicans MET3 promoter downstream of the HAH1 cassette of pFA-HAH1. The plasmid contains a HAH1 cassette consisting of His1-ARG4-HIS1 markers. The MET3 promoter is meant for conditional gene expression in C. albicans. The nucleotide sequence of the plasmid corresponds with the EMBL Nucleotide Sequence Database accession number HQ455521.1. Other name of the plasmid is pFA-HAH1-MET3prom. |
| EMBL Accession number: | HQ455521.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/03/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Dr K. Ganesan(1). It was constructed by Dr S. Paul(1). (1) CSIR Institute of Microbial Technology, Chandigarh, India |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pFA-HAH1-MET3P (LMBP 11187) is available at BCCM/GeneCorner. The plasmid was deposited by Dr K. Ganesan. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.