Last data update: 24 January 2024 16:39 CET
Plasmid name: pFAJ1702 (LMBP 9764)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9764.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Rhizobium sp. NGR234 symbiotic plasmid stability locus AB (spsAB, y4dLM) |
| Promoter: | Escherichia coli lac operon promoter Transposon Tn5 neomycin resistance gene promoter (neo) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Escherichia coli tryptophan synthase α subunit terminator (trpA); synthetic sequence |
| Selection marker: | Ampicillin (amp) Tetracycline (tet) |
| Replicon: | Broad-host-range plasmid RK2 vegetative replication origin Broad-host-range plasmid RK2/RP4 origin of transfer (oriT) |
| Host range: | Escherichia coli Gram-negative bacterial strains |
| Parental clone: | pFAJ1706 |
| Further information: | The plasmid was created by introducing a multiple cloning site into the pFAJ1706 vector as a HindIII-BamHI fragment. This plasmid is a stable RK2-derived cloning vector that can be used for the study of genes in a wide background of gram-negative bacteria. The plasmid RK2 trfA gene encodes two polypeptide products P382 and P285 of 382 and 285 amino acids respectively, using a different initiation codon within the same reading frame. The resulting protein is essential for vegetative replication. The plasmid carries the Rhizobium sp. NGR234 spsAB postsegregational killer locus to ensure stable maintenance of the plasmid. The multiple cloning site of the plasmid is protected against read-through transcriptional activity of vector sequences by the trpA transcriptional terminator. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/01/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr J. Michiels(1). (1) Centre of Microbial and Plant Genetics, Department of Microbial and Molecular Systems, KU Leuven, Leuven, Belgium |
| Plasmid reference: | Dombrecht et al., Mol. Plant Microbe Interact. 14 (2001), 426-430 [PMID: 11277442] [DOI: 10.1094/MPMI.2001.14.3.426] |
| Related plasmid reference: | Weinstein et al., J. Bacteriol. 174 (1992), 7486-7489 [PMID: 1429472] [DOI: 10.1128/jb.174.22.7486-7489.1992] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pFAJ1702 (LMBP 9764) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Michiels and was published in Dombrecht et al., 2001. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.