Last data update: 24 January 2024 16:39 CET
Plasmid name: pFB-Neo-FLAG-COP/Pseudo-ICE (LMBP 5291)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human caspase-1 dominant negative inhibitor pseudo-ICE cDNA (hCOP1) FLAG epitope tag; N-terminal |
| Promoter: | Moloney mouse leukemia virus long terminal repeat (5' MMLV-LTR) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Moloney mouse leukemia virus long terminal repeat (3' MMLV-LTR) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; preferably recombination-deficient strains Mammalian cells |
| Parental clone: | pFB-Neo |
| Further information: | The nucleotide sequence of the pFB-Neo vector was obtained from the Stratagene website (version 114002). Other name of the plasmid is pFBneo-FLAG-COP/Pseudo-ICE. |
| EMBL Accession number: | AF367017, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 06/06/2006 |
| Sequence detail: | pFB-Neo Multiple Cloning Site Region GTC.GAC.GAA.TTC.GGA.TCC.TCG.AGC.GGC.CGC SalI EcoRI BamHI XhoI NotI |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Vandenabeele(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Lamkanfi et al., J. Biol. Chem. 279 (2004), 51729-51738 [PMID: 15383541] |
| Related plasmid reference: | Miller, in 'Retroviruses', Cold Spring Harbor Laboratory Press, NY (1997), 437-473 [ISSN/ISBN: 0879695714] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 XL1-Blue MRF' |
| Host reference: | Jerpseth et al., Strategies 5 (1992), 81-83 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 in E. coli; L2 in mammalian cells |
| Other culture collection numbers: | - |
| Related website: | http://www.iresite.org/IRESite_web.php?page=view&entry_id=140 |
Refer in your Materials and Methods: |
pFB-Neo-FLAG-COP/Pseudo-ICE (LMBP 5291) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Vandenabeele and was published in Lamkanfi et al., 2004. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.