Last data update: 24 January 2024 16:39 CET
Plasmid name: pFBR-neo (LMBP 1347)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p1347.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Finkel-Biskis-Reilly mouse osteosarcoma virus gag-fos fusion gene (FBR-MSV p75, gag-fos) Escherichia coli colicin E1 immunity protein cDNA (imm, GeneID 8656959) |
| Promoter: | Herpes simplex virus (HSV) thymidine kinase promoter (TK) pBR322 P1 promoter Finkel-Biskis-Reilly mouse osteosarcoma virus long terminal repeat (5' FBR-MSV LTR) |
| Ribosome binding site: |
- |
| Terminator: | Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Finkel-Biskis-Reilly mouse osteosarcoma virus long terminal repeat (3' FBR-MSV LTR) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pHSG272 |
| Further information: | The plasmid was constructed by inserting a large (approx. 7.5 kb) HindIII fragment, containing the FBR-MSV sequence, flanked by rat celllular DNA, into the unique HindIII site of pHSG272. The FBR-MSV coding sequence consists of a fusion of mouse gag and fos (p75). The gag-fos fusion contains the 5' and 3' truncated fos gene (24 and 98 AA resp.). The fos part contains 2 in-frame deletions and encodes several different amino acids as compared to wild type c-fos. The fox part contains non specified cellular rat and/or mouse DNA (Van Beveren, Virology 135). The plasmid is used for oncogenic transformation. The P1 promoter is artificially created by ligation of two DNA fragments to create pBR322. The sequence of the rat cellular DNA is unknown. The nucleotide sequence of the FBR-MSV corresponds with the EMBL Nucleotide Sequence Database accession number K02712.2. |
| EMBL Accession number: | K02712.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 08/05/1995 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr R. Müller(1) (1) Institute of Molecular Biology and Tumor Research, Philipps University Marburg, Marburg, Germany |
| Plasmid reference: | Jenuwein et al., Cell 41 (1985), 629-637 [PMID: 2985286] |
| Related plasmid reference: | Curran et al., Mol. Cell. Biol. 3 (1983), 914-921 [PMID: 6306448] Van Beveren et al., Virology 135 (1984), 229-243 [PMID: 6203215] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pFBR-neo (LMBP 1347) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr R. Müller Institute of Molecular Biology and Tumor Research, Philipps University Marburg, Marburg, Germany and was published in Jenuwein et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.