Last data update: 24 January 2024 16:39 CET
Plasmid name: pFGA6 (LMBP 1943)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p1943.gb
(View with Genome Compiler) p1943.txt p1943.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Aspergillus niger glucoamylase genomic DNA (glaA); mutated sequence |
| Promoter: | Aspergillus niger glucoamylase promoter (glaA) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Fungi; integrative |
| Parental clone: | pFGA2 |
| Further information: | This is the same plasmid as pFGA2, except for a unique StuI site created by site-specific mutagenesis. Upon StuI digestion of pFGA6, a blunt end is created directly behind the Lys Arg codons of the A. niger glaA propeptide sequence. This vector can only be co-transformed in fungi. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727605.1. The nucleotide sequence of the fungal part of the plasmid (= nucleotide position 414 to 3834) corresponds with the EMBL Nucleotide Sequence Database accession number AM270061.1, except for the mutated fragment. Other name of the plasmid is pDC1. |
| EMBL Accession number: | AM270061.1, view at EMBL, GenBank, DDBJ LT727605.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 27/02/2013 |
| Sequence detail: | Sequence before (pFGA2) and after (pFGA6) site-specific mutagenesis:
pFGA2 pFGA6
Ser Lys Arg Ala Thr Ser Lys Arg Pro Thr
5' TCC.AAG.CGC.GCG.ACC 3' 5' TCC.AAG.AGG.CCT.ACC 3'
StuI |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: HindII, HindIII, HindIII/NaeI, NheI, SalI and StuI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by D. Carrez(1) and Prof. Dr R. Contreras(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Carrez et al., Gene 94 (1990), 147-154 [PMID: 2258049] |
| Related plasmid reference: | Boel et al., EMBO J. 3 (1984), 1097-1102 [PMID: 6203744] Boel et al., EMBO J. 3 (1984), 1581-1585 [PMID: 6204865] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pFGA6 (LMBP 1943) is available at BCCM/GeneCorner. This plasmid was deposited by D. Carrez and Prof. Dr R. Contreras and was published in Carrez et al., 1990. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.