Last data update: 24 January 2024 16:39 CET
Plasmid name: pFastBac1-hGABBR2-Clover-His (LMBP 10439)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p10439.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human gamma-aminobutyric acid type B receptor subunit 2 cDNA (GABBR2, GPR51, GABABR2, GPRC3B, HG20, GeneID 9568) Human rhinovirus 3C protease cleavage site (PreScission site, PRS); C-terminal Aequorea victoria green fluorescent protein DNA (GFP); enhanced yellow-green variant (Clover), C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Autographa californica nuclear polyhedrosis virus (AcNPV) polyhedrin promoter (PH) Escherichia coli plasmid R388 class 1 integron Pc promoter (PcS) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Gentamicin (Gm) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli Insect cells; e.g. Sf9 cells |
| Parental clone: | pFastBac1 |
| Further information: | The plasmid was constructed by cloning the human GABBR2 coding sequence with the C-terminal Clover reporter gene into the pFastBac1 vector. The plasmid contains Tn7 transposon elements, allowing site-directed transposition in the E. coli DH10Bac strain. The C-terminal Clover reporter gene and the C-terminal His-tag can be removed from the encoded protein through the HRV-3C protease cleavage site or PreScission site. Other name of the plasmid is pFastBac1-GBR2-Clover-His. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/08/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr S. Savvides(1)(2). It was constructed by Dr. I. Ramou(1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pFastBac1-hGABBR2-Clover-His (LMBP 10439) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr S. Savvides. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.