Last data update: 24 January 2024 16:39 CET
Plasmid name: pG20-6xHis-Blp (LMBP 12720)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p12720.gb |
| Sequence analysis results Genecorner: |
NGS: g12-gc-may2021.fasta |
| Cloned DNA: |
Histidine tag (His-tag); C-terminal |
| Promoter: | Cauliflower mosaic virus 35S promoter (CaMV 35S) Arabidopsis thaliana polyubiquitin 10 promoter (UBQ10) |
| Ribosome binding site: |
- |
| Terminator: | Cauliflower mosaic virus 35S terminator (CaMV 35S) Arabidopsis thaliana heat shock protein 18.2 terminator (HSP18.2) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) Phosphinotricin (basta) |
| Replicon: | Escherichia coli plasmid pMB1 origin Escherichia coli plasmid pSa origin |
| Host range: | Escherichia coli Plant cells |
| Parental clone: | pG20-mVenus-Blp |
| Further information: | The plasmid was constructed by replacing the mVenus coding sequence in pG20-mVenus-Blp with a His-tag sequence. This plasmid is intended for cloning in plants, using the constitutive AtUBQ10 promoter in combination with the AtHSP18.2 terminator, resulting in strong, tissue-independent expression. It is part of an updated pGREEN-based plasmid set. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MT896413.1. Other name of the plasmid is pG20_6xHis_Blp. |
| EMBL Accession number: | MT896413.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 20/08/2021 |
| Authenticity test: | This plasmid has been fully sequenced, but the NGS data still needs to be implemented. The NGS data showed an additional fragment of approx. 1340 nucleotides compared to the EMBL sequence. According to the depositor, this fragment has no influence on the functionality of the plasmid. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by A. Pratt(1) and Prof. Dr H. Kunz(1). (1) School of Biological Sciences, Washington State University, USA |
| Plasmid reference: | Pratt et al., MicroPubl. Biol. 10 (2020), 17912 [PMID: 33274321] [DOI: 10.17912/micropub.biology.000317] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | Addgene 159712 |
Refer in your Materials and Methods: |
pG20-6xHis-Blp (LMBP 12720) is available at BCCM/GeneCorner. This plasmid was deposited by A. Pratt and Prof. Dr H. Kunz and was published in Pratt et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.