Last data update: 24 January 2024 16:39 CET
Plasmid name: pG20-FLAG-Hyg (LMBP 12721)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p12721.gb |
| Sequence analysis results Genecorner: |
NGS: h01-gc-may2021.fasta |
| Cloned DNA: |
FLAG epitope tag; C-terminal |
| Promoter: | Cauliflower mosaic virus 35S promoter (CaMV 35S) Arabidopsis thaliana polyubiquitin 10 promoter (UBQ10) |
| Ribosome binding site: |
- |
| Terminator: | Cauliflower mosaic virus 35S terminator (CaMV 35S) Arabidopsis thaliana heat shock protein 18.2 terminator (HSP18.2) |
| Selection marker: | Neomycin (neo; kanamycin (kan)) Hygromycin (hyg) |
| Replicon: | Escherichia coli plasmid pMB1 origin Escherichia coli plasmid pSa origin |
| Host range: | Escherichia coli Plant cells |
| Parental clone: | pG20-mVenus-Hyg |
| Further information: | The plasmid was constructed by replacing the mVenus coding sequence in pG20-mVenus-Hyg with FLAG-epitope sequence This plasmid is intended for cloning in plants, using the constitutive AtUBQ10 promoter in combination with the AtHSP18.2 terminator, resulting in strong, tissue-independent expression. It is part of an updated pGREEN-based plasmid set. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number MT896414.1. Other name of the plasmid is pG20_FLAG_Hyg. |
| EMBL Accession number: | MT896414.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 20/08/2021 |
| Authenticity test: | The plasmid has been fully sequenced but the NGS data still needs to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by A. Pratt(1) and Prof. Dr H. Kunz(1). (1) School of Biological Sciences, Washington State University, USA |
| Plasmid reference: | Pratt et al., MicroPubl. Biol. 10 (2020), 17912 [PMID: 33274321] [DOI: 10.17912/micropub.biology.000317] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | Addgene 159713 |
Refer in your Materials and Methods: |
pG20-FLAG-Hyg (LMBP 12721) is available at BCCM/GeneCorner. This plasmid was deposited by A. Pratt and Prof. Dr H. Kunz and was published in Pratt et al., 2020. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.