Last data update: 24 January 2024 16:39 CET
Plasmid name: pG9m-SkPCASP (LMBP 10452)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccoglossus kowaleski paracaspase cDNA (PCASP, MALT1 homolog) |
| Promoter: | Escherichia coli chloramphenicol acetyltransferase promoter (cat) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 early transcription terminator (T7Te) Phage T3 early transcription terminator (T3Te) Escherichia coli tonB-P14 terminator (tonB) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pG9m |
| Further information: | The plasmid was constructed by cloning the synthetic S. kowaleski PCASP coding sequence into the pG9m vector. The acorn worm paracaspase represents an important evolutionary check point in the evolution of MALT1: the acorn worm is a hemichordate - a non-chordate deuterostome with good sequence information. Conserved features in a non-chordate (ambulacraria) deuterostome tells us which features were present in the last common deuterostome ancestor. Is synthetically generated by Gen9 |
| EMBL Accession number: | - |
| Latest sequence update: | 18/07/2019 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Staal et al., Front. Immunol. 9 (2018), 1136 [PMID: 29881386] [DOI: 10.3389/fimmu.2018.01136] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pG9m-SkPCASP (LMBP 10452) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert and was published in Staal et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.