Last data update: 24 January 2024 16:39 CET
Plasmid name: pGAD-mMyD88 (LMBP 5651)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5651.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse myeloid differentiation primary response gene 88 cDNA (Myd88, GeneID 17874); complete CDS, Myd88 long (Myd88l) Saccharomyces cerevisiae GAL4 activation domain (GALad) |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | pGAD424 |
| Further information: | Yeast 2-Hybrid construction. The mouse myeloid differentiation primary response gene 88 cDNA (Myd88), encoding an adaptor protein involved in TLR and IL-1R induced activation of NF-κB, was picked up from the mouse macrophage cell line Mf4/4 and cloned in frame with the N-terminal GALad. The nucleotide sequence of the mouse Myd88 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number U84409.1. |
| EMBL Accession number: | U84409.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 21/01/2008 |
| Sequence detail: | 1 2 3 4 5
Forward primer: 5'G.GAA.TTC.CCA.TGG.GCG.GCC.GCG.ATG.TCT.GCG.GGA.GAC.CCC.CGC 3'
EcoRI NotI
296 295 290
Reverse primer: 5'CGCCCTGCAGCTCGAG.TCA.GGG.CAG.GGA.CAA.AGC.CTT.GGC.AAG 3'
PstI |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Janssens(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Burns et al., J. Exp. Med. 197 (2003), 263-268 [PMID: 12538665] Janssens et al., Trends Biochem. Sci. 27 (2002), 474-482 [PMID: 12217523] Janssens et al., FEBS Lett. 548 (2003), 103-107 [PMID: 12885415] Janssens et al., Curr. Biol. 12 (2002), 467-471 [PMID: 11909531] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGAD-mMyD88 (LMBP 5651) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Janssens and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.