Last data update: 24 January 2024 16:39 CET
Plasmid name: pGAD424-mABIN-2mEE (LMBP 4403)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p4403.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae GAL4 activation domain (GALad) Mouse A20-binding inhibitor of NF-κB activation 2 cDNA (ABIN-2, TNIP2); mutated sequence |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | pGAD424-mABIN-2; pCAGGS-E-mABIN-2mEE |
| Further information: | The plasmid was constructed by inserting the NotI-BglII fragment from pCAGGS-E-mABIN-2mEE, containing the mutated mouse A20-binding inhibitor of NF-κB activation 2 cDNA (mABIN-2), into the NotI-BglII opened pGAD424-mABIN-2 vector. As a result, the coding sequence of the mutated mABIN-2 cDNA is fused in phase to the Saccharomyces cerevisiae GAL4 activation domain (GALad). The fusion protein will be targeted to the nucleus by the nuclear localization signal of the SV40 large T-antigen that has been added to the GAL4 activation domain sequence. As compared to the wt mABIN-2 cDNA, the codons at position 302 (Gln) and 303 (Gln) were replaced by 2 Glu codons. The nucleotide sequence of the mABIN-2 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AJ304865.1. Other name of the plasmid is pGAD424-mABIN-2(QQ302EE). |
| EMBL Accession number: | AJ304865.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 26/07/2001 |
| Sequence detail: | Nucleotide sequence of the mutated mABIN-2 cDNA:
wild type: 5' ATG.CTA.GAA.CAG.CAG.ATT.CTT.GCT 3'
Met Leu Glu Gln Gln Ile Leu Ala
mutated : 5' ATG.CTA.GAA.GAG.GAG.ATT.CTT.GCT 3'
Met Leu Glu Glu Glu Ile Leu Ala
^ ^
^: Mutated nucleotide.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Van Huffel et al., J. Biol. Chem. 276 (2001), 30216-30223 [PMID: 11390377] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGAD424-mABIN-2mEE (LMBP 4403) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.