Last data update: 24 January 2024 16:39 CET
Plasmid name: pGADhCH3E (LMBP 4080)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4080.gb
(View with Genome Compiler) p4080.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae GAL4 activation domain (GALad) Human immunoglobulin γ3 cDNA (hIG3); CH3 domain of the heavy chain E-tag; C-terminal |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | pGAD424 |
| Further information: | The plasmid was constructed by inserting an EcoRI/SalI fragment, containing the human immunoglobulin γ3 heavy chain (CH3) fragment with a C-terminal E-tag, between the EcoRI and SalI sites of pGAD424. pGADhCH3E is useful to screen for interacting human CH3 domains in the MATCHMAKER‚ Two Hybrid system from Clontech…. |
| EMBL Accession number: | - |
| Latest sequence update: | 09/01/2001 |
| Sequence detail: | Nucleotide sequence at the fusion between GALad and the human CH3 coding sequence:
------ GALad ------> ------- CH3 ------->
5' ... AAC.CCA.AAA.AAA.GAG.ATC.GAA.TTC.CCG.GGG.ATC.CAG.CCC.CGA.GAA.CCA ... 3'
Asn Pro Lys Lys Glu Ile Glu Phe Pro Gly Ile Gln Pro Arg Glu Pro
EcoRI AvaI BamHI
Nucleotide sequence at the C-terminal E-tag:
------- CH3 -------> ---------------------- E-tag ------
5' ... CTG.TCT.CCG.GGT.AAA.GCG.GCC.GCA.GGT.GCG.CCG.GTG.CCG.TAT.CCG.GAT.CCG.
Leu Ser Pro Gly Lys Ala Ala Ala Gly Ala Pro Val Pro Tyr Pro Asp Pro
NotI BamHI
-------------->
CTG.GAA.CCG.CGT.GCC.GCA.TAG.ACTGTTGAAAGTTGT ... 3'
Leu Glu Pro Arg Ala Ala +++
+++: Termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BanI, EcoRI/PstI, EcoRV/SalI, HindII and KpnI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr R. Schoonjans(1) (2) and Prof. Dr J. Grooten(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGADhCH3E (LMBP 4080) is available at BCCM/GeneCorner. This plasmid was deposited by Dr R. Schoonjans and Prof. Dr J. Grooten . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.