Last data update: 24 January 2024 16:39 CET
Plasmid name: pGADmBclxL (LMBP 4771)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4771.gb
(View with Genome Compiler) p4771.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse B-cell leukemia/lymphoma 2-like 1 cDNA (BclxL, Bcl2l1); long form Saccharomyces cerevisiae GAL4 activation domain (GALad) |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) LEU2; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; leu2(-) |
| Parental clone: | pGAD424 |
| Further information: | The plasmid was constructed as follows: mouse B-cell leukemia/lymphoma x long form cDNA (mBclxL) was isolated from a L929r2 cDNA library and inserted into the EcoRI site of pGAD424. As a result, the mBclxL coding sequence was fused in frame to the S. cerevisiae GAL4 activation domain (GALad). The fusion protein will be targeted to the nucleus by the nuclear localization signal of the SV40 large T-antigen that has been added to the GAL4 activation domain sequence. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727033.1.1 The nucleotide sequence of mBclxL corresponds with the Genbank accession number U51278.1. |
| EMBL Accession number: | U51278.1, view at EMBL, GenBank, DDBJ LT727033.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 20/11/2003 |
| Sequence detail: | Nucleotide sequence at the fusion of the GALad and the mouse BclxL coding sequence:
GALad ------------->
881
5' ... AAC.CCA.AAA.AAA.GAG.ATC.GAA.TTC.GGC.ACG.AGA.CTG.GTT.GAG.CCC.ATC.TCT.
Asn Pro Lys Lys Glu Ile Glu Phe Gly Thr Arg Leu Val Glu Pro Ile Ser
EcoRI
------ mBclxL ----->
1
ATT.ATA.AAA.ATG.TCT.CAG.AGC.AAC ... 3'
Ile Ile Lys Met Ser Gln Ser Asn
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI and NcoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by M. Van Gurp(1) (2) and Prof. Dr P. Vandenabeele (1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGADmBclxL (LMBP 4771) is available at BCCM/GeneCorner. This plasmid was deposited by M. Van Gurp and Prof. Dr P. Vandenabeele . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.