Last data update: 24 January 2024 16:39 CET
Plasmid name: pGAP1-GFP (LMBP 9756)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae general amino acid permease cDNA (GAP1) Aequorea victoria green fluorescent protein DNA (GFP); pH-sensitive S65T mutant, C-terminal |
| Promoter: | Saccharomyces cerevisiae general amino acid permease promoter (GAP1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae general amino acid permease terminator (GAP1) |
| Selection marker: | Ampicillin (amp) Saccharomyces cerevisiae URA3; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae centromeric region 6 (CEN6)/ S. cerevisiae autonomously replicating sequence ARSH4 |
| Host range: | Escherichia coli Saccharomyces cerevisiae; ura3(-) |
| Parental clone: | pFL38 |
| Further information: | The plasmid is derived from pFL38 and contains the S. cerevisiae GAP1 promoter, the GAP1 coding sequence in frame with the C-terminal mutated GFP coding sequence and the GAP1 terminator. The plasmid contains the S65T-GFP mutant, whose fluorescence is known to be pH sensitive (Kneen et al., 1998). |
| EMBL Accession number: | - |
| Latest sequence update: | 12/01/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr G. Van Zeebroeck(1)(2) and Prof. Dr J. Thevelein(1)(2). It was constructed by Dr M. Rubio-Texeira(1)(2). (1) Department of Molecular Microbiology, VIB, Leuven, Belgium (2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, Department of Biology, KU Leuven, Leuven, Belgium |
| Plasmid reference: | Rubio-Texeira et al., Nat. Chem. Biol. 8 (2012), 400-408 [PMID: 22388927] [DOI: 10.1038/nchembio.910] |
| Related plasmid reference: | Kneen et al., Biophys. J. 74 (1998), 1591-1599 [PMID: 9512054] [DOI: 10.1016/S0006-3495(98)77870-1] Bonneaud et al., Yeast 7 (1991), 609-615 [PMID: 1767589] [DOI: 10.1002/yea.320070609] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGAP1-GFP (LMBP 9756) is available at BCCM/GeneCorner. This plasmid was deposited by Dr G. Van Zeebroeck and Prof. Dr J. Thevelein and was published in Rubio-Texeira et al., 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.