Last data update: 24 January 2024 16:39 CET
Plasmid name: pGBKT7-unr (LMBP 5261)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5261.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human cold shock domain containing E1 cDNA (CSDE1, D1S155E, UNR, upstream of NRAS) Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Myc epitope; N-terminal |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Phage T7 gene 10 promoter (T7g10) Escherichia coli β-lactamase promoter (amp) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli ampicillin resistance gene (amp) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Neomycin (neo; kanamycin (kan)) TRP1; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) Phage f1 origin |
| Host range: | Escherichia coli Saccharomyces cerevisiae; trp1(-) |
| Parental clone: | pET21d-UNR; pGBKT7 |
| Further information: | The plasmid was created by cloning the NcoI-XmaI digested PCR amplification product obtained from pET21d-UNR into the NcoI-XmaI opened pGBKT7 vector. The pGBKT7 vector expresses proteins fused to amino acids 1-147 of the GAL4 DNA binding domain. The plasmid is meant for expression in yeast. The nucleotide sequence of the CSDE1 coding sequence corresponds with Genbank Accession number NM_007158.5. Other name of the plasmid is pGBKT7 UNR. |
| EMBL Accession number: | NM_007158.5, view at GenBank |
| Latest sequence update: | 09/03/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Y. Bruynooghe(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Tinton et al., Biochem. J. 385 (2005), 155-163 [PMID: 15330758] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGBKT7-unr (LMBP 5261) is available at BCCM/GeneCorner. This plasmid was deposited by Y. Bruynooghe and Prof. Dr R. Beyaert and was published in Tinton et al., 2005. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.