Last data update: 24 January 2024 16:39 CET
Plasmid name: pGBT9-CezN (LMBP 5570)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5570.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human OTU deubiquitinase 7B cDNA (OTUD7B, CEZANNE); N-terminal fragment Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) |
| Promoter: | Saccharomyces cerevisiae alcohol dehydrogenase promoter (ADH1) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Saccharomyces cerevisiae alcohol dehydrogenase terminator (ADH1) Saccharomyces cerevisiae 2 micron plasmid (2μ) FLP terminator |
| Selection marker: | Ampicillin (amp) TRP1; auxotrophic |
| Replicon: | Escherichia coli plasmid pMB1 origin Saccharomyces cerevisiae 2 micron plasmid origin (2μ); incl. region conferring stability (STB) |
| Host range: | Escherichia coli Saccharomyces cerevisiae; trp1(-) |
| Parental clone: | pGBT9 |
| Further information: | The plasmid was constructed by cloning an N-terminal fragment of the human OTUD7B coding sequence, consisting of codons 1 up to and including 443, into the pGBT9 vector. The plasmid is meant for yeast two hybrid screening. The nucleotide sequence of the human OTUB7B coding sequence corresponds with the Genbank accession number NM_020205.3. |
| EMBL Accession number: | NM_020205.3, view at GenBank |
| Latest sequence update: | 13/05/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr P. Evans(1). (1) Department of Infection, Immunity and Cardiovascular Disease, Medical School, University of Sheffield, Sheffield, UK |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGBT9-CezN (LMBP 5570) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Evans. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.