Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEM-TAX1BP1-S (LMBP 3781)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p3781.gb
(View with Genome Compiler) p3781.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human Tax1 binding protein 1 cDNA (TAX1BP1, T6BP, TXBP151, GeneID 8887); small form (TAX1BP1-S) |
| Promoter: | Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEM-11ZF(+); pSV/TXBP15-1-AS |
| Further information: | The plasmid was constructed by inserting the XhoI-EcoRI fragment of pSV/TXBP15-1-AS, containing the splice variant of the human Tax1 binding protein 1 cDNA (TAX1BP1-S), into the EcoRI-XhoI opened pGEM-11ZF(+) vector. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727387.1. Referring to the pCAGGS-TAX1BP1-S sequence analysis results of the Department of Biomedical Molecular Biology (Ghent University, Belgium), the human TAX1BP1-S coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number U33821.2, except for the nucleotides CG at position 147-148 being substituted by GC, resulting in H49Q and V50L mutations, and nucleotide T at position 1203 being substituted by C, resulting in a silent mutation. These differences were confirmed by Dr K-T. Jeang (NIH, Bethesda, Maryland, USA) who deposited pSV/TXBP15-1 (LMBP 3514) and pSV/TXBP15-1-AS (LMBP 3515). Other name of the plasmid is pGEM11ZF(+)TXBP151-S. |
| EMBL Accession number: | U33821.2, view at EMBL, GenBank, DDBJ LT727387.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 04/02/2002 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII/XhoI, EcoRI, EcoRI/XhoI, NdeI/XbaI and NgoMIV. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). It was constructed by D. De Valck(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Valck et al., Oncogene 18 (1999), 4182 - 4190 [PMID: 10435631] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEM-TAX1BP1-S (LMBP 3781) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert and was published in De Valck et al., 1999. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.