Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEM-hTGFB1C2S2-2 (LMBP 3251)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human transforming growth factor beta 1 cDNA (TGFB1, TGFB, DPD1, GeneID 7040); mutated fragment |
| Promoter: | Escherichia coli lac operon promoter Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEM11-Zf(+); pRK5-B1EC2S2 |
| Further information: | The plasmid was constructed by inserting the SacI/HindIII fragment of pRK5-B1EC2S2, containing the 3' part of the human TGFB1 coding sequence, between the SacI and HindIII sites of the polylinker of pGEM-11Zf(+). The human TGFB1 coding sequence contains C223S and C225S mutations (C2S2) and has an engineered starting codon. These mutations result in expression of human TGFB1 that is largely biologically active. The plasmid can be used for the production of single-stranded RNA probes (riboprobes). When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. Other name of the plasmid is pGEM11RK5C2S2RP2. |
| EMBL Accession number: | - |
| Latest sequence update: | 12/04/2004 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by K. Staes(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | Deposit door Katrien voor Annick Keirsebilck Number 275 in U7 collection. |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEM-hTGFB1C2S2-2 (LMBP 3251) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.