Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEME6L (LMBP 1975)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | pgeme6l.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) cDNA; light chain (E6L) |
Promoter: | Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
Ribosome binding site: |
- |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pGEM1; pSV23SE6L |
Further information: | The plasmid was constructed by inserting the HindIII-SalI fragment of pSV23SE6L, encoding the light chain E6L of the mouse anti-hPLAP monoclonal antibody E6(IgG2b,κ) into the unique HindIII-SalI vector fragment of pGEM1. The 3' untranslated region of E6L still contains 15 dC-nucleotides of the dG/dC tail. |
EMBL Accession number: | - |
Latest sequence update: | 28/03/1990 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by P. Casneuf(1) and Prof. Dr W. Fiers(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEME6L (LMBP 1975) is available at BCCM/GeneCorner. This plasmid was deposited by P. Casneuf and Prof. Dr W. Fiers. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.