Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEMIL6gIL2f3 (LMBP 2323)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p2323.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 2 genomic DNA (IL2, IL-2, TCGF, GeneID 3558); fragment |
| Promoter: | Human interleukin 6 promoter (IL6) Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEMIL6gIL2f1; pSV2L5 |
| Further information: | The plasmid was constructed by ligating the SV40 polyA fragment from pSV2L5 as a HindIII-HpaI (nucleotide position 3576) into the HindIII-HindII opened pGEMIL6gIL2f1. The human interleukin 2 genomic DNA (hIL2) of this plasmid contains the first exon (coding partially for the signal sequence), the first intron, the second exon and part of the third exon (up to the XbaI site). Note that the second intron of the hIL2 gene is not present. So, the second exon and part of the third exon are contiguous (this junction region is derived from the cDNA sequence). The sequence of the genomic hIL2 part was taken from Genbank (Release 55; Locus: HUMIL2). It differs in one nucleotide (nucleotide position 362) from the incompletely determined hIL2 sequence as described by Degrave et al., 1983. The nucleotide sequence of the genomic hIL6 DNA corresponds with the EMBL Nucleotide Sequence Database accession number AC073072.11. However, the C nucleotide at promoter position -174 (polymorphism mutant) was changed into a G nucleotide (wild type). |
| EMBL Accession number: | AC073072.11, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 30/04/2004 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by V. Vande Vijver(1) and Prof. Dr G. Haegeman(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Degrave et al., EMBO J. 2 (1983), 2349-2353 [PMID: 6321163] |
| Related plasmid reference: | De Wet et al., Mol. Cell. Biol. 7 (1987), 725-737 [PMID: 3821727] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEMIL6gIL2f3 (LMBP 2323) is available at BCCM/GeneCorner. This plasmid was deposited by V. Vande Vijver and Prof. Dr G. Haegeman and was published in Degrave et al., 1983. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.