Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEMT-mCad10 (LMBP 6646)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6646.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Mouse transmenbrane glycoprotein cadherin 10 cDNA (Cdh10); 3' fragment |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pGEM-T |
| Further information: | pGEMT-mCad10 contains a 3' fragment of the mouse cadherin 10 cDNA (192 codons and the 3' UTR), that can be used as a probe. The nucleotide sequence of the mouse cadherin 10 cDNA corresponds with the EMBL Nucleotide Sequence Database accession number AF183946.1, except for 2 nucleotides of the 3'UTR. |
| EMBL Accession number: | AF183946.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/06/2010 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by Dr K. Vanhalst(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Koen Vanhalst (2003) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEMT-mCad10 (LMBP 6646) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy and was published in Koen Vanhalst, 2003. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.