Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEMhBCTNf3 (LMBP 4365)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4365.gb
(View with Genome Compiler) p4365.txt p4365.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | Human β-catenin promoter (BCTN, CTNNB1) Escherichia coli lac operon promoter Phage SP6 promoter Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pSEAPBhBCTNf3; pGEM-11Zf(+) |
| Further information: | The plasmid was constructed by inserting the 5,6 kb EcoRI fragment of the human CTNNB1 gene, containing 3,4 kb of the 5' flanking region, the CTNNB1 promoter sequence, the non-coding exon 1 (5' untranslated region) and 1 kb of intron 1, into the unique EcoRI site of pGEM-11Zf(+). The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727198.1. The nucleotide sequence of the human CTNNB1 promoter corresponds with the EMBL Nucleotide Sequence Database accession number X89448.1. The nucleotide sequence of the 5' flanking region, the 5' untranslated region and the first intron of the human CTNNB1 gene corresponds with the Genbank accession number NT_022517.18.. The nucleotide sequence of the pGEM-11Zf(+) backbone corresponds with the EMBL Nucleotide Sequence Database accession number X65313.2. Other name of the plasmid is pBCTNpromotor. |
| EMBL Accession number: | X89448.1, view at EMBL, GenBank, DDBJ NT_022517.18, view at EMBL, GenBank, DDBJ X65313.2, view at EMBL, GenBank, DDBJ LT727198.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 10/05/2012 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, BglII, Eco47III/EcoRV, EcoRI and NdeI/PstI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr F. Nollet(1) (2) and Prof. Dr F. Van Roy(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Nollet et al., Genomics 32 (1996), 413-424 [PMID: 8838805] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | LMBP 03788 |
Refer in your Materials and Methods: |
pGEMhBCTNf3 (LMBP 4365) is available at BCCM/GeneCorner. This plasmid was deposited by Dr F. Nollet and Prof. Dr F. Van Roy and was published in Nollet et al., 1996. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.