Last data update: 24 January 2024 16:39 CET
Plasmid name: pGEX-GST-mUBAN-ER/AA (LMBP 8084)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | not available |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Mouse A20-binding inhibitor of NF-κB activation 1 cDNA (ABIN-1, TNIP1); polyubiquitin binding domain (UBAN); mutant Schistosoma japonicum Sj26 antigen glutathione S-transferase gene (GST) Thrombin recognition site; N-terminal Escherichia coli lac repressor gene (lacI) Escherichia coli lac Z gene (lacZ); fragment, incl. α part |
Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (tac) Escherichia coli lac repressor promoter; mutant (lacI(q)) Escherichia coli lac operon promoter |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli |
Parental clone: | pGEX-2T |
Further information: | The plasmid contains the polyubiquitin binding domain (UBAN) of mouse ABIN-1; ranging from amino acid 465 up to and including 520. The mouse ABIN-1 gene contains the DFxxER motif. ER/AA mutations in this motif were introduced by site-directed mutagenesis. The nucleotide sequence of the wild type mouse ABIN-1 cds corresponds with the EMBL Nucleotide Sequence Database accession number AJ242778.1. Other name of the plasmid is pGEX GST mUBAN ER/AA. |
EMBL Accession number: | AJ242778.1, view at EMBL, GenBank, DDBJ |
Latest sequence update: | 24/09/2013 |
Sequence detail: | Primers used to introduce the ER/AA mutations: 483 485 489 490 495 496 forward: 5' GAA.GAG.GAC.TTC.CAG.AGG.GCA.GCG.AGT.GAC.CGT.GAA.CGC.ATG 3' Glu Glu Asp Phe Gln Arg Ala Ala Ser Asp Arg Glu Arg Met ^ ^^ reverse: 5' CAT.GCG.TTC.ACG.GTC.ACT.CGC.TGC.CCT.CTG.GAA.GTC.CTC.TTC 3' ^^ ^ wt sequence of mABIN-1: 483 485 489 490 495 496 5' ... GAA.GAG.GAC.TTC.CAG.AGG.GAA.CGG.AGT.GAC.CGT.GAA.CGC.ATG ... 3' Glu Glu Asp Phe Gln Arg Glu Arg Ser Asp Arg Glu Arg Met ^: mutated nucleotide. Punctuation indicates reading frame. |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr I. Dikic(1). (1) Institute of Biochemistry II, Goethe University School of Medicine, Frankfurt, Germany |
Plasmid reference: | - |
Related plasmid reference: | Wagner et al., Oncogene 27 (2008), 3739-3745 [PMID: 18212736] [DOI: 10.1038/sj.onc.1211042] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 DH5α |
Host reference: | Focus 8 (1986), 9 |
Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Cultivation remark: | - |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGEX-GST-mUBAN-ER/AA (LMBP 8084) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr I. Dikic. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.