Last data update: 24 January 2024 16:39 CET
Plasmid name: pGal4-VP16 (LMBP 4708)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p4708.gb
(View with Genome Compiler) p4708.txt p4708.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Saccharomyces cerevisiae GAL4 DNA-binding domain (GALbd) Herpes simplex viral protein VP16 transcriptional activation domain (VPad, VP16ad) |
| Promoter: | Rous sarcoma virus long terminal repeat (RSV-LTR) Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pABgal |
| Further information: | The plasmid contains the transcriptional activation domain of the Herpes simplex protein VP16 (VPad) C-terminal fused to the S. cerevisiae GALbd domain composed of the first 147 codons from GAL4. The GALbd-VPad fusion protein can be used as a bait protein in two-hybrid screening. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727167.1. The nucleotide sequence upstream of GALbd in pABgal (from nucleotide position 1381 up to 1950 of pABgal) has been analysed by BCCM/GeneCorner. Other name of the plasmid is pABgal4-vp16. |
| EMBL Accession number: | LT727167.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/09/2003 |
| Sequence detail: | See page 2.
Nucleotide sequence at the start of the Saccharomyces cerevisiae GAL4 DNA-binding domain:
---------- GALbd ---------->
2
5' ... AGAGTTGATATTCACTG.ATG.GAC.TCC.CAG.CAG.CCA.GAT.CTG.AAG.CTA.CTG.TCT.TCT.ATC.GAA ... 3'
Met Asp Ser Gln Gln Pro Asp Leu Lys Leu Leu Ser Ser Ile Glu
*** BglII
Nucleotide sequence at the GALbd-VPad fusion:
-------- GALbd --------> --------- linker ---------> --------- VPad -------->
147 413
5' ... AGA.CAG.TTG.ACT.GTA.TCG.CCG.GAA.TTC.CCG.GGG.ATC.TGG.GCC.CCC.CCG.ACC.GAT.GTC ... 3'
Arg Gln Leu Thr Val Ser Pro Glu Phe Pro Gly Ile Trp Ala Pro Pro Thr Asp Val
HindII EcoRI AvaI ApaI
SmaI
Nucleotide sequence at the end of the Herpes simplex viral protein VP16 transcriptional activation domain:
--------------------------- VPad -------------------------->
490
5' ... CAG.ATG.TTT.ACC.GAT.GCC.CTT.GGA.ATT.GAC.GAG.TAC.GGT.GGG.TAG.GGGGCGCGACCGGACCC ... 3'
Gln Met Phe Thr Asp Ala Leu Gly Ile Asp Glu Tyr Gly Gly +++
StyI
***: Start codon.
+++: Termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: ApaI, BamHI, BglI/XhoI, EcoRI/PstI, NcoI/PvuI, StuI, StyI and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr M.L. Schmitz(1). (1) Department of Chemistry and Biochemistry, University of Bern, Switzerland |
| Plasmid reference: | Schmitz et al., EMBO J. 10 (1991), 3805-3817 [PMID: 1935902] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pGal4-VP16 (LMBP 4708) is available at BCCM/GeneCorner. This plasmid was deposited by Dr M.L. Schmitz and was published in Schmitz et al., 1991. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.