Last data update: 24 January 2024 16:39 CET
Plasmid name: pHLSec-hCSF-1R-D1-D3-HIS (LMBP 9804)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human colony stimulating factor 1 receptor cDNA (C-FMS, CD115, CSFR, CSF1R); fragment Histidine tag (His-tag); C-terminal |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pHLsec |
| Further information: | The plasmid was constructed by cloning an N-terminal fragment of the human CSF1R coding sequence, containing codons 1 up to and including 296, into the EcoRI-KpnI opened pHLSec vector. The plasmid encodes for extracellular domains 1 to 3 of the human CSF1R protein. The nucleotide sequence of the human CSF1R coding sequence corresponds with the Genbank accession number NM_001288705.1. Other name of the plasmid is PhlSec_hCSF-1R_D1-D3_HIS. |
| EMBL Accession number: | NM_001288705.1, view at GenBank |
| Latest sequence update: | 23/05/2016 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr S. Savvides (1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) Inflammation Research Center, VIB, Ghent, Belgium |
| Plasmid reference: | Felix et al., Structure 21 (2013), 528-539 [PMID: 23478061] [DOI: 10.1016/j.str.2013.01.018] |
| Related plasmid reference: | Felix et al., Structure 23 (2015), 1621-1631 [PMID: 26235028] [DOI: 10.1016/j.str.2015.06.019] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHLSec-hCSF-1R-D1-D3-HIS (LMBP 9804) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr S. Savvides and was published in Felix et al., 2013. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.