Last data update: 24 January 2024 16:39 CET
Plasmid name: pHLSec-hMBP-hDRD2-Avi-His (LMBP 10425)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human myelin basic protein cDNA (MBP, GeneID 4155) Human dopamine receptor D2 cDNA (DRD2, GeneID 1813) Avidin biotinylation sequence (AviTag); C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Chicken β-actin/rabbit β-globin hybrid promoter (AG) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pHLsec |
| Further information: | The plasmid was constructed by cloning the human DRD2 coding sequence, preceded by the human MBP coding sequence and followed by an AviTag, into the pHLsec vector. Other name of the plasmid is pHLSec-MBP-D2-Avi-His. |
| EMBL Accession number: | - |
| Latest sequence update: | 16/08/2017 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr S. Savvides(1)(2). It was constructed by Dr. I. Ramou(1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
| Plasmid reference: | - |
| Related plasmid reference: | Aricescu et al., Acta Crystallogr. D Biol. Crystallogr. 62 (2006), 1243-1250 [PMID: 17001101] [DOI: 10.1107/S0907444906029799] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHLSec-hMBP-hDRD2-Avi-His (LMBP 10425) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr S. Savvides. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.