Last data update: 24 January 2024 16:39 CET
Plasmid name: pHLsec-hIL9(19-144)-T117M-Avi-His (LMBP 12813)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human interleukin 9 cDNA (IL9, IL-9, HP40, P40, GeneID 3578); mutated fragment Avidin biotinylation sequence (AviTag); C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Chicken β-actin/rabbit β-globin hybrid promoter (AG) Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pHLsec-hIL9(19-144)-Avi-His |
| Further information: | The plasmid was constructed by introducing a T117M mutation into the human IL9 coding sequence of pHLsec-hIL9(19-144)-Avi-His. The plasmid is intended for transient expression of the human IL9 fragment in mammalian cells. The C-terminal AviTag and C-terminal His-tag can be removed from the encoded protein via a CASP3 cleavage site. Other name of the plasmid is pHLsec_hIL-9(T117M)_AVI_HIS. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/11/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr S. Savvides(1)(2). It was constructed by Dr T. De Vos(1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHLsec-hIL9(19-144)-T117M-Avi-His (LMBP 12813) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr S. Savvides. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.