Last data update: 24 January 2024 16:39 CET
Plasmid name: pHM6-TRABID (LMBP 5575)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p5575.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human TRAF-binding domain cDNA (TRABID, ZRANB1) Influenza HA epitope encoding the haemagglutinin tagging peptide; N-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Phage SP6 promoter |
| Ribosome binding site: |
- |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pHM6 |
| Further information: | pHM6-TRABID is an eukaryotic expression vector for hTRABID, in frame with a N-terminal HA epitope. As the termination codon of hTRABID is included, the C-terminal His-tag is not expressed. The nucleotide sequence of the hTRABID cDNA corresponds with the EMBL Nucleotide Sequence Database Accession number AJ252060.1 except for the T-nucleotide in position 1560 of the hTRABID cds which was replaced by C, creating an additional HindIII site, which was confirmed by restriction analysis. This nucleotide change does not cause an amino acid change. Other name of the plasmid is pHM6-HATRABID. |
| EMBL Accession number: | AJ252060.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 23/11/2007 |
| Sequence detail: | The borders of the insert have been sequenced:
|-> TRABID
| HA epitope | | 1 2 3 4 5
5'...ATG.TAC.CCA.TAC.GAC.GTC.CCA.GAC.TAC.GCT.GGA.AGC.TTG.GGT.ACC.ATG.TCA.GAA.CGT.GGA.ATT...
HindIII KpnI
705 709
.....GAT.GAT.GAA.GAT.GAA.TGA.GAATTCGCGGCCGC...3'
EcoRI NotI |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI/HindIII, NdeI, NcoI and PvuI. The plasmid contains an additional PvuI site located around nucleotide position 5400. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr Paul Evans(1). (1) Cardiovascular Science Subdivision, National Heart and Lung Institute, School of Medicine, Imperial College London, UK |
| Plasmid reference: | Evans et al., Biochem. J. 357 (2001), 617-623 [PMID: 11463333] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pHM6-TRABID (LMBP 5575) is available at BCCM/GeneCorner. This plasmid was deposited by Dr Paul Evans and was published in Evans et al., 2001. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.