Last data update: 24 January 2024 16:39 CET
Plasmid name: pIM162 (LMBP 9309)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Yersinia enterocolitica specific yopT chaperone gene (sycT) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pIM154; pBluescriptIIKS- |
| Further information: | The plasmid was constructed by cloning the Y. enterocolitica sycT CDS from pIM154 as a SalI/XbaI fragment into the pBluescriptIIKS- vector. The plasmid expresses SycT under the control of the E. coli lac operon promoter. Other names of the plasmid are pBlue: Plac-sycT+ and pBluescriptII SK- Plac sycT. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/07/2014 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr M. Iriarte(2). (1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium (2) Microbial Pathogenesis Unit, Institute of Cellular and Molecular Pathology, Catholic University of Louvain, Brussels, Belgium |
| Plasmid reference: | Iriarte et al., Mol. Microbiol. 29 (1998), 915-929 [PMID: 9723929] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 LK111 |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIM162 (LMBP 9309) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Iriarte et al., 1998. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.