Last data update: 24 January 2024 16:39 CET
Plasmid name: pIRES-SEAP (LMBP 8505)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
Human placental alkaline phosphatase gene (PLAP, ALPP); mutant encoding a secreted protein (SEAP) |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Phage T7 gene 10 promoter (T7g10) Simian virus 40 early promoter (SV40 early) Escherichia coli lac operon promoter |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Bovine growth hormone polyadenylation signal (BGH polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| Parental clone: | pSEAP2; pIRESneo2 |
| Further information: | The plasmid was constructed by isolating the human SEAP coding sequence from pSEAP2 as a NheI/HpaI fragment and cloning it into the pIRESneo2 vector. |
| EMBL Accession number: | - |
| Latest sequence update: | 07/04/2023 |
| Authenticity test: | The plasmid has been fully sequenced but the NGS data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Buss(1). (1) Cambridge Institute for Medical Research, University of Cambridge, UK |
| Plasmid reference: | Chibalina et al., Methods Enzymol. 438 (2008), 11-24 [PMID: 18413238] [DOI: 10.1016/S0076-6879(07)38002-6] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIRES-SEAP (LMBP 8505) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Buss and was published in Chibalina et al., 2008. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.