Last data update: 24 January 2024 16:39 CET
Plasmid name: pIRES2-EGFP-PDGF-C (LMBP 8467)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p8467.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Rattus norvegicus platelet derived growth factor C (Pdgfc) Aequorea victoria green fluorescent protein DNA (GFP); enhanced red-shifted variant (EGFP) |
| Promoter: | Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer Simian virus 40 early promoter (SV40 early) Escherichia coli β-lactamase promoter (amp) |
| Ribosome binding site: |
Internal ribosome entry site (IRES) of the encephalomyocarditis virus (EMCV) polyprotein gene |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) Herpes simplex virus (HSV) thymidine kinase polyadenylation signal (TK polyA) |
| Selection marker: | Neomycin (neo; G418; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pIRES2-EGFP |
| Further information: | The plasmid was constructed by PCR amplifying the rat Pdgfc cDNA and cloning it as a BglII/SalI fragment into the BglII/SalI opened pIRES2-EGFP vector. The nucleotide sequence of the rat Pdgfc cDNA corresponds with the Genbank accession number NM_031317.1. |
| EMBL Accession number: | NM_031317.1, view at GenBank |
| Latest sequence update: | 17/06/2013 |
| Sequence detail: | Primers used to isolate the rat Pdgfc cds:
forward 5′ TGCATAGATCTGCCACC.ATG.CTC.CTC.CTC.GGC.CTC
BglII ***
reverse 5′ GACTGTCGAC.TTA.CCC.TTC.TGT.GTT.TCC.TCT.ACA
SalI +++
***: starting codon.
+++: termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr B. Tang(1). (1) Department of Vascular Surgery, The Second Affiliated Hospital of Chongqing Medical University, Chongqing, China |
| Plasmid reference: | Tang et al., Plasmid 69 (2013), 195-201 [PMID: 22935743] [DOI: 10.1016/j.plasmid.2012.07.006] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pIRES2-EGFP-PDGF-C (LMBP 8467) is available at BCCM/GeneCorner. This plasmid was deposited by Dr B. Tang and was published in Tang et al., 2013. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.