Last data update: 24 January 2024 16:39 CET
Plasmid name: pJ3ECAD (LMBP 5155)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p5155.gb
(View with Genome Compiler) p5155.txt p5155.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human cadherin 1 cDNA (CDH1, E-cad, UVO, CD324, GeneID 999) |
| Promoter: | Simian virus 40 early promoter (SV40 early) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pJ3omega; pSM1; pSM9; pDR2ECAD2 |
| Further information: | The plasmid was constructed as follows: fragments from human CDH1, isolated from pSM1, pSM9 and pDR2ECAD2, were combined to reconstruct full-length, human CDH1 cDNA, which was inserted between the XbaI and filled-in HindIII sites of pJ3omega (ATCC). This plasmid contains the SV40 small t-antigen splicing signal. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727097.1. The borders of the insert and the ligation zones of the fragments were sequenced at the Department of Biomedical Molecular Biology (Ghent University, Belgium). The nucleotide sequence of the human CDH1 cDNA was obtained from the EMBL Nucleotide Sequence Database accession number Z13009.1. Other name of the plasmid is pJ3ECad (cl1). |
| EMBL Accession number: | Z13009.1, view at EMBL, GenBank, DDBJ LT727097.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 15/02/2006 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, KpnI, NcoI, SacI, XbaI and XhoI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr F. Van Roy(1) (2). It was constructed by K. Staes(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Nawrocki-Raby et al., Am. J. Pathol. 163 (2003), 653-661 [PMID: 12875984] |
| Related plasmid reference: | Morgenstern et al., Nucleic Acids Res. 18 (1990), 1068 [PMID: 2156225] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pJ3ECAD (LMBP 5155) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr F. Van Roy and was published in Nawrocki-Raby et al., 2003. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.