Last data update: 24 January 2024 16:39 CET
Plasmid name: pJRD184 (LMBP 1503)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | pjrd184.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pJRD158 |
| Further information: | This plasmid is a pBR322 derivative with several extra unique restriction sites and a somewhat higher copy number (due to a single nucleotide difference in the incompatibility region). The HindIII does not always inactivate the tetracycline gene. |
| EMBL Accession number: | - |
| Latest sequence update: | 13/03/1992 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Davison(1). (1) Department of Genetics, University of Dublin, Dublin, Ireland |
| Plasmid reference: | Heusterspreute et al., Gene 39 (1985), 299-304 [PMID: 3005114] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + tetracycline (10 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pJRD184 (LMBP 1503) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Davison and was published in Heusterspreute et al., 1985. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.