Last data update: 24 January 2024 16:39 CET
Plasmid name: pKai-FcgRI-VHH-M2e-VHH (LMBP 12095)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Llama anti-mouse Fcɣ receptor I (FcɣRI) VHH single domain antibody Llama anti-influenza A matrix protein 2 (M2), ectodomain (M2e) VHH single domain antibody Histidine tag (His-tag); C-terminal Saccharomyces cerevisiae α-mating factor 1 gene (MFα1, GeneID 855914); prepro secretion signal sequence (ppMF) |
| Promoter: | Pichia pastoris alcohol oxidase 1 promoter (AOX1) Saccharomyces cerevisiae translation elongation factor 1α promoter (TEF1α) Synthetic prokaryotic EM7 promoter |
| Ribosome binding site: |
- |
| Terminator: | Pichia pastoris alcohol oxidase 1 terminator (AOX1) Saccharomyces cerevisiae iso-1-cytochrome C terminator (CYC1) |
| Selection marker: | Bleomycin (bleo; zeomycin (zeo; Zeocin); phleomycin (phleo)) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Pichia pastoris; integrative |
| Parental clone: | pKai61 |
| Further information: | The plasmid was constructed by PCR amplifying the Llama anti-mouse FcɣRI and the Llama anti-influenza M2e VHH encoding sequences and cloning them as fusion genes into the XhoI/SpeI opened pKai61 vector. The vectors is meant to be linearized by PmeI and transformed into P. pastoris. Other name of the plasmid is pKai_FcgRI VHH-M2e VHH. |
| EMBL Accession number: | - |
| Latest sequence update: | 17/08/2020 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr X. Saelens(1)(2). It was constructed by Dr D. De Vlieger(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium |
| Plasmid reference: | De Vlieger et al., Front. Immunol. 10 (2019), 2920 [PMID: 31921179] [DOI: 10.3389/fimmu.2019.02920] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + zeocin (25 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pKai-FcgRI-VHH-M2e-VHH (LMBP 12095) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr X. Saelens and was published in De Vlieger et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.