Last data update: 24 January 2024 16:39 CET
Plasmid name: pLHM36-TaCtnnb1-ARM-MalE (LMBP 12586)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Trichoplax adhaerens catenin beta 1 cDNA (Ctnnb1); fragment Escherichia coli maltose ABC transporter periplasmic binding protein cDNA (malE, GeneID 948538) DEVD caspase cleavage site Histidine tag (His-tag); N-terminal |
| Promoter: | Phage λ major leftward promoter (λ PL) Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pLHM36; pLT32 |
| Further information: | The plasmid was constructed by ligating part of the pLHM36 vector, containing the E. coli malE cds, and part of the pLT32 vector, containing the pMB1 ori and the ampicillin resistance cassette, together with a PCR fragment containing the ARM domain of the T. adhaerens Ctnnb1 coding sequence. Other name of the plasmid is Ta_Ctnnb1_ARM_MalE |
| EMBL Accession number: | - |
| Latest sequence update: | 18/10/2021 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by K. Staes(1)(2) and Prof. Dr F. Van Roy(1)(2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLHM36-TaCtnnb1-ARM-MalE (LMBP 12586) is available at BCCM/GeneCorner. The plasmid was deposited by K. Staes and Prof. Dr F. Van Roy. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.