Last data update: 24 January 2024 16:39 CET
Plasmid name: pLHX32-hRIP4 (LMBP 7899)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p7899.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human receptor-interacting serine-threonine kinase 4 cDNA (RIPK4, RIP4, ANKRD3, ANKK2, DIK, PKK) Histidine tag (His-tag); N-terminal Escherichia coli thioredoxin (TRX, trxA) |
| Promoter: | Phage T3 promoter Phage T7 gene 10 promoter (T7g10) Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pLHX32 |
| Further information: | The plasmid was constructed by cloning the human RIPK4 coding sequence into the pLHX32 vector. This low copy number plasmid can be used for IPTG-inducible expression of His-tagged human RIPK4 after transformation into an E. coli strain containing a compatible piCA plasmid. The His-tagged RIPK4 is not fused to trxA. Expression of human RIPK4 can be offered by the Protein Service Facility (PSF), Inflammation Research Center, VIB-Ghent University, Belgium. Please contact BCCM/GeneCorner for your protein expression request or refer to BCCM/GeneCorner when contacting PSF directly. The nucleotide sequence of the human RIP4 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number BC110617.1. Other names of the plasmid are pLT32HXhRIP4 and pLHXhRIP4 kl 1a dd16_06. |
| EMBL Accession number: | BC110617.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 24/06/2015 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. De Groote(1) (2) and Prof. Dr W. Declercq(1) (2). (1) Inflammation Research Center, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | PhD thesis Philippe De Groote (2012) |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | LMBP 05343 |
Refer in your Materials and Methods: |
pLHX32-hRIP4 (LMBP 7899) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. De Groote and Prof. Dr W. Declercq and was published in Philippe De Groote, 2012. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.