Last data update: 24 January 2024 16:39 CET
Plasmid name: pLJ6 (LMBP 9313)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p9313.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Yersinia enterocolitica secretion protein P gene (yscP) |
| Promoter: | Escherichia coli L-arabinose operon promoter (araB, araBAD, BAD) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli L-ribulokinase gene (araB); optimized sequence |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pBAD/Myc-HisA |
| Further information: | The plasmid was constructed by cloning the Y. enterocolitica yscP CDS into the NcoI/EcoRI opened pBAD/Myc-HisA vector. Other names of the plasmid are pBAD:yscPwt+ and pBAD/Myc-HisA-yscP. |
| EMBL Accession number: | - |
| Latest sequence update: | 03/07/2014 |
| Sequence detail: | Primers used to amplify the yscP CDS:
Forward: 5' GATCCC.ATG.GCC.AAT.AAA.ATC.ACC.ACT.CGT
***
NcoI
Reverse: 5' GATCGAATTC.TTA.TTC.TTC.AGC.CTC.CCA.CTC
EcoRI +++
***: starting codon.
+++: termination codon.
Punctuation indicates reading frame. |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr G. Cornelis(1). It was constructed by Dr L. Journet(2). (1) Research Unit in Microorganism Biology, University of Namur, Namur, Belgium (2) Biozentrum, University of Basel, Switzerland |
| Plasmid reference: | Journet et al., Science 302 (2003), 1757-1760 [PMID: 14657497] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 LK111 |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLJ6 (LMBP 9313) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr G. Cornelis and was published in Journet et al., 2003. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.