Last data update: 24 January 2024 16:39 CET
Plasmid name: pLK15 (LMBP 1912)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
plk15.gb
(View with Genome Compiler) plk15.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage fd gene 3 (g3) |
| Promoter: | Phage λ major leftward promoter (λ PL) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage fd gene 3 |
| Terminator: | - |
| Selection marker: | Kanamycin (kan) |
| Replicon: | Escherichia coli plasmid ColE1 origin |
| Host range: | Escherichia coli; use strains with a cI function |
| Parental clone: | pPLa2311 |
| Further information: | Gene 3 of fd phage was first cloned as an AluI-AluI fragment into the HindII site of pUR222; then the cloned gene was inserted in pPLa2311 as a PstI-EcoRI fragment. No repression of P1 is needed; the fd-3 protein is not toxic and can be constitutively expressed. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727597.1. |
| EMBL Accession number: | LT727597.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 11/10/1989 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI, EcoRI and HindII. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr P. Bross(1). (1) University of Konstanz |
| Plasmid reference: | Bross et al., J. Gen. Microbiol. 134 (1988), 461-471 [PMID: 3171545] |
| Related plasmid reference: | Beck et al., Nucleic Acids Res. 5 (1978), 4495-4503 [PMID: 745987] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLK15 (LMBP 1912) is available at BCCM/GeneCorner. This plasmid was deposited by Dr P. Bross and was published in Bross et al., 1988. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.