Last data update: 24 January 2024 16:39 CET
Plasmid name: pLNR2 (LMBP 1518)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | plnr2.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Phage λ N gene Phage λ cI temperature-sensitive repressor gene (cIts, cI857) |
| Promoter: | Phage λ major rightward promoter (λ PR) Phage λ major leftward promoter (λ PL) Phage λ promoter for repressor maintenance (λ PRM) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage λ N gene |
| Terminator: | - |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pAT153cI857 |
| Further information: | The plasmid was constructed by cloning a λ BglII-BamHI fragment (containing the PL promoter and coding region of the N gene) in the BamHI site from pAT153cI857. The orientation of the inserted N gene is opposite to that of the cI857 temperature sensitive repressor gene. The λ PR promoter is controlled by the cI857 temperature sensitive repressor that is cloned on the same plasmid. The λ PR promoter can be used for the expression of fragments containing a ribosome binding site functional in E. coli. Expression of the λ N gene is controlled by PL promoter and cI857 repressor. The length of the G/C tails after the cI857 gene is not known. |
| EMBL Accession number: | - |
| Latest sequence update: | 25/03/2011 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | Mertens et al., Biotechnology 13 (1995), 175-179 [PMID: 9634760] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLNR2 (LMBP 1518) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr E. Remaut and was published in Mertens et al., 1995. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.