Last data update: 24 January 2024 16:39 CET
Plasmid name: pLT10T3-His-TAT-mABIN-1f-E (LMBP 4230)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p4230.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human immunodeficiency virus (HIV) trans-activator of transcription cDNA (TAT); protein transduction domain Mouse A20-binding inhibitor of NF-κB activation 1 cDNA (ABIN-1, TNIP1); minimal NF-κB inhibiting fragment Histidine tag (His-tag); N-terminal E-tag; C-terminal |
Promoter: | Phage λ major leftward promoter (λ PL) Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
Terminator: | Phage T7 gene 10 terminator (T7g10) Phage fd terminator |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
Host range: | Escherichia coli |
Parental clone: | - |
Further information: | This plasmid was constructed by inserting an XmaI-NotI PCR amplicon, containing a fusion gene between a fragment of the HIV TAT cDNA and the minimal NF-κB inhibiting mouse ABIN-1 cDNA fragment that consists of nucleotides 1446 to 1919 of the coding sequence, flanked by the N-terminal His-tag and the C-terminal E-tag. The plasmid is meant for procaryotic expression of the mABIN gene. Other name of the plasmid is pLT10T3His-ABIN(1446-1919)-E. |
EMBL Accession number: | - |
Latest sequence update: | 30/03/2009 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | - |
Related plasmid reference: | Nagahara et al., Nat. Med. 12 (1998), 1449-1452 [PMID: 9846587] Schwarze et al., Science 285 (1999), 1569-1572 [PMID: 10477521] |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061(λ) |
Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLT10T3-His-TAT-mABIN-1f-E (LMBP 4230) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.