Last data update: 24 January 2024 16:39 CET
Plasmid name: pLT10TH-F-MALT1Cd80-GyrB (LMBP 6105)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | p6105.gb |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Human MALT1 paracaspase cDNA (MALT1, MLT, PCASP1, GeneID 10892); fragment Escherichia coli DNA gyrase subunit B (gyrB); fragment Enterokinase cleavage site (EK); N-terminal FLAG epitope tag; N-terminal Histidine tag (His-tag); N-terminal |
| Promoter: | Phage λ major leftward promoter (λ PL) Phage T7 gene 10 promoter (T7g10) Phage T3 promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10) |
| Terminator: | Phage fd terminator Phage T7 gene 10 terminator (T7g10) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pLT10TH; pCD-LCK-HLH-F-MALT1Cd80-GyrB |
| Further information: | The plasmid was constructed by isolating the FLAG-tagged minimal active domain of human MALT1, fused to the E. coli Gyrase B dimerization domain, from pCD-LCK-HLH-F-MALT1Cd80-GyrB, and cloning it into the pLT10TH vector. This clone is used for mapping of minimal active domain of MALT1 for proteolytic activity. The nucleotide sequence of the human MALT1 coding sequence corresponds with the EMBL Nucleotide Sequence Database accession number AF130356.2. |
| EMBL Accession number: | AF130356.2, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 07/04/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr J. Staal(1) (2) and Prof. Dr R. Beyaert(1) (2). (1) UGent-VIB Center for Inflammation Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli B BL21-CodonPlus-RIL[pT7POL26] |
| Host reference: | - |
| Helper plasmid: | pT7POL26 |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLT10TH-F-MALT1Cd80-GyrB (LMBP 6105) is available at BCCM/GeneCorner. This plasmid was deposited by Dr J. Staal and Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.