Last data update: 24 January 2024 16:39 CET
Plasmid name: pLT10lth minusTAT CrmAmut (LMBP 3998)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | not available |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Cowpox virus cytokine response modifier A cDNA (crmA); mutated sequence |
| Promoter: | Phage T7 gene 10 promoter (T7g10) |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
| Parental clone: | pLT10 lth minus TAT CASP-11 c/s Etag; pCAGGS CrmA mut |
| Further information: | The plasmid was constructed by PCR amplifying the crmA cds from pCAGGS CrmA mut, and ligating it as a NotI/KpnI fragment into the NotI/KpnI opened pLT10 lth minus TAT CASP-11 c/s Etag vector. The nucleotide sequence of the wild type crmA cDNA corresponds with the EMBL Nucleotide Sequence Database accession number M14217.1. |
| EMBL Accession number: | M14217.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 09/10/2013 |
| Sequence detail: | Primers used to amplify the crmA mutant cds: Forward : 5' CGG GTA CCC GAT ATC TTC AGG GAA ATC GCA TC Reverse : 5' AAG GAA AAT AGC GGC CGC ATT AGT TGT TGG AGA GCA ATA TCT AC |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr R. Beyaert(1) (2). (1) Department for Molecular Biomedical Research, VIB, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061(λ) |
| Host reference: | Mertens et al., Gene 164 (1995), 9-15 [PMID: 7590329] |
| Related host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLT10lth minusTAT CrmAmut (LMBP 3998) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr R. Beyaert . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.