Last data update: 24 January 2024 16:39 CET
Plasmid name: pLV-EF1a-msfGFP-T2A-mCherry-NLS_mPGK-Puro (LMBP 13903)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Synthetic green fluorescent protein DNA (GFP); monomeric superfolder GFP (msfGFP) Thosea asigna virus 2A peptide (T2A) Discosoma sp. red fluorescent protein DNA (DsRed1); mutated monomeric variant (mCherry) Nuclear localization signal (NLS); C-terminal |
| Promoter: | Rous sarcoma virus/human immunodeficiency virus hybrid long terminal repeat (RSV/HIV-1 5' LTR) Escherichia coli lac operon promoter Phage T3 promoter Human elongation factor 1α promoter (EF1α) |
| Ribosome binding site: |
- |
| Terminator: | Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) Puromycin (puro) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli Mammalian cells |
| EMBL Accession number: | - |
| Latest sequence update: | 07/11/2023 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by T. Van de Steene(1)(2) and Prof. Dr S. Eyckerman(1)(2). (1) VIB-UGent Center for Medical Biotechnology, VIB, Ghent, Belgium (2) Department of Biomolecular Medicine, Ghent University, Ghent, Belgium |
| Plasmid reference: | Delhaye et al., Cell Rep. Methods (2023), submitted [DOI: 10.1101/2023.11.03.565112] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5αT1R |
| Host reference: | - |
| Related host reference: | Killmann et al., J. Bacteriol. 178 (1996), 6913-6920 [PMID: 8955314] [DOI: 10.1128/jb.178.23.6913-6920.1996] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLV-EF1a-msfGFP-T2A-mCherry-NLS_mPGK-Puro (LMBP 13903) is available at BCCM/GeneCorner. This plasmid was deposited by T. Van de Steene and Prof. Dr S. Eyckerman and was published in Delhaye et al., 2023. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.