Last data update: 24 January 2024 16:39 CET
Plasmid name: pLacDN4HIFNGma (LMBP 1635)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p1635.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Human interferon γ cDNA (IFNG) Escherichia coli lac Z gene (lacZ); starting at the 9th codon Escherichia coli lac Y gene (lacY) Escherichia coli lac A gene (lacA) |
Promoter: | Phage λ major leftward promoter (λ PL) |
Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli tryptophan operon attenuator peptide (trp) |
Terminator: | - |
Selection marker: | Ampicillin (amp) |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli; use strains with a cI function, cIts for PL controlled expression |
Parental clone: | pLacDN4HIFNG; pSP64 |
Further information: | In this plasmid, the SalI-EcoRI fragment from the polylinker of pLacDN4HIFNG was replaced by the SalI-EcoRI fragment from the polylinker of pSP64. This plasmid bears an in-phase nonsense (amber) codon in the hIFNG-lacZ fusion protein. This amber codon is very poorly suppressible. The chromosomal lacZ gene must be inactivated for detection of β-galactosidase activity of the hIFNG-lacZ fusion protein. Other names of the plasmid are pDN4G1 and pLacDN4HIFNG1. |
EMBL Accession number: | - |
Latest sequence update: | 25/10/1989 |
Sequence detail: | Nucleotide sequence around the hIFNG-lacZ junction: 139 amber 5' CGA.GGT.CGA.CTC.TAG.AGG.ATC.CCC.GGG.CGA.GCT.CGA.ATT.CCC.GGG.GAT.CCC. SalI XbaI BamHI SmaI SacI EcoRI SmaI BamHI XmaI XmaI AA9 of lacZ GTC.GTT 3' Punctuation indicates reading frame. Nonsense (amber) codon. |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by E. Sablon(1) and Prof. Dr E. Remaut(1). (1) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
Plasmid reference: | PhD thesis Erwin Sablon (1990) |
Restricted distribution: | - BCCM MTA |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061[pcI857] |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Helper plasmid: | pcI857 |
Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) + kanamycin (50 μg/ml) |
Cultivation temperature: | 28°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLacDN4HIFNGma (LMBP 1635) is available at BCCM/GeneCorner. This plasmid was deposited by E. Sablon and Prof. Dr E. Remaut and was published in Erwin Sablon, 1990. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.