Last data update: 24 January 2024 16:39 CET
Plasmid name: pLacSO32 (LMBP 6279)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner non-core plasmid |
| Depositor's sequence: | - |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
Escherichia coli outer membrane protein A cDNA (ompA, tolG, GeneID 945571); signal sequence Histidine tag (His-tag); N-terminal Escherichia coli DNA-binding transcriptional repressor LacI cDNA (lacI, GeneID 945007) |
| Promoter: | Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Phage T7 gene 10 terminator (T7g10) Phage fd terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Phage f1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pLH36 |
| Further information: | The plasmid was constructed by cloning the E. coli lacI coding sequence, the E. coli ompA signal sequence and the f1 ori into the pLH36 vector. The plasmid contains an enterokinase restriction site. |
| EMBL Accession number: | - |
| Latest sequence update: | 26/03/2009 |
| Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by J. Haustraete(1) (2). (1) Protein Core, VIB-UGent Center for Inflammation Research, Ghent, Belgium (2) Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium |
| Plasmid reference: | - |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + 2% glu + ampicillin (100 μg/ml) |
| Cultivation temperature: | 28°C |
| Biosafety level: | L1 |
| Cultivation remark: | Glucose is necessary to repress the lac promoter during cell growth. The promoter can be induced with IPTG for protein production. Subcultivation: LB-Lennox-2glu = LB-Lennox + 2% glucose Expression: in LB-Lennox-0,2glu = LB-Lennox + 0,2 % glucose |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pLacSO32 (LMBP 6279) is available at BCCM/GeneCorner. This plasmid was deposited by J. Haustraete . |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.